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tpc2 a1 n  (MedChemExpress)
94
MedChemExpress tpc2 a1 n
Tpc2 A1 N, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpc2 a1 p  (MedChemExpress)
90
MedChemExpress tpc2 a1 p
( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
Tpc2 A1 P, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpc2 agonist tpc2 a1 n  (MedChemExpress)
94
MedChemExpress tpc2 agonist tpc2 a1 n
( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
Tpc2 Agonist Tpc2 A1 N, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpc2 ion channel  (Proteostasis Therapeutics)
90
Proteostasis Therapeutics tpc2 ion channel
( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
Tpc2 Ion Channel, supplied by Proteostasis Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpc2  (Novus Biologicals)
93
Novus Biologicals tpc2
( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
Tpc2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b16f10lucwt, tpc2 ko or rab7a ko cells  (Envigo)
90
Envigo b16f10lucwt, tpc2 ko or rab7a ko cells
( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
B16f10lucwt, Tpc2 Ko Or Rab7a Ko Cells, supplied by Envigo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

Journal: bioRxiv

Article Title: NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation

doi: 10.64898/2026.04.13.718294

Figure Lengend Snippet: ( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

Article Snippet: The TPC2 or TPC1 channel currents were elicited by perfusion of PtdIns( , )P2 diC8 (Echelon Biosciences #P-3058), TPC2-A1-P (MedChemExpress #HY-131615 or Sigma-Aldrich #SML3700), or TPC2-A1-N (MedChemExpress #HY-131614 or Sigma-Aldrich #SML3562) on the cytosolic side in inside-out recordings and whole endolysosomal recordings, or of TPC2-A1-P on the extracellular side in cell-attached recordings.

Techniques: Western Blot, Construct

Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.

Journal: bioRxiv

Article Title: NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation

doi: 10.64898/2026.04.13.718294

Figure Lengend Snippet: Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.

Article Snippet: The TPC2 or TPC1 channel currents were elicited by perfusion of PtdIns( , )P2 diC8 (Echelon Biosciences #P-3058), TPC2-A1-P (MedChemExpress #HY-131615 or Sigma-Aldrich #SML3700), or TPC2-A1-N (MedChemExpress #HY-131614 or Sigma-Aldrich #SML3562) on the cytosolic side in inside-out recordings and whole endolysosomal recordings, or of TPC2-A1-P on the extracellular side in cell-attached recordings.

Techniques: Knock-Out, Expressing